Bufotes sitibundus

Literary information about the batrachofauna of the Orenburg nature reserve is incomplete and contradictory. Data on the specific composition and abundance of amphibians in the Orenburg Nature State Reserve at its five cluster territories by the results of our 2015–2020 inventory are presented. The previously published materials about amphibians on the cluster territories “Talovskaya Steppe”, “Burtinskaya Steppe” and “Aytuarskaya Steppe” have been supplemented.

Distribution of cryptic species of green toads (Bufotes viridis and B. sitibundus) in Dagestan still understudied. We used the nuclear (microsatellite BM224) and mitochondrial (fragment of the CytB gene) markers characterized by species-specific alleles and haplotypes, respectively. Alleles of B. viridis predominated in toad populations in the plains in the north of the republic, while B.